Journal: PLoS ONE
Article Title: Extended LTA, TNF, LST1 and HLA Gene Haplotypes and Their Association with Rubella Vaccine-Induced Immunity
doi: 10.1371/journal.pone.0011806
Figure Lengend Snippet: A 3,145 kb segment of the extended HLA gene region is shown. The LTA, TNF, and LST1 loci encoded in the class III region of the HLA complex in relation to HLA-B and HLA-DRB1 loci. The positions of single nucleotide polymorphisms (SNPs) selected from LTA [rs2857602 (31641357), rs2857708 (31641585), rs915654 (31646476), rs2844482 (31647746), rs1041981 (31648763), rs1799964 (31650287), rs1799724 (31650461)], TNF [rs1800629 (31651010)], and LST1 [rs2256965 (31663109), rs2256974 (31663371)] gene families and used for haplotype estimation are shown. Targeted sets of extended haplotypes used in this study: (1) haplotypes containing the three class I HLA loci (A-C-B) and the set of ten-SNP haplotypes, (2) haplotypes containing ten-SNP haplotypes and the class II HLA loci (DRB1-DQA1-DQB1-DPA1-DPB1), and (3) haplotypes containing class I HLA loci (A-C-B), the set of ten-SNP haplotypes, and alleles of class II HLA loci (DRB1-DQA1-DQB1-DPA1-DPB1). Note: the figure is not to scale.
Article Snippet: Class II HLA typing was performed with high resolution DRB1 SSP, DQA1 SSP, DQB1 SSP, DPA1 SSP, and DPB1 SSP Unitray® typing kits with the entire locus on a single tray (Invitrogen).
Techniques: